研究方向

1.进行清道夫抗体、纳米抗体、双特异抗体、抗体药物偶联物等新型抗体研究，获得原创性抗体分子，应用于感染性疾病和肿瘤的治疗。

2.建立CAR-T细胞治疗平台，在三阴性乳癌、脑胶质瘤、肝癌等实体瘤治疗方面进行开拓性研究，经联合治疗后在三阴性乳腺癌治疗中取得突破性进展。

3.围绕基于抗体药物研发的关键技术环节，建立了一个从抗体筛选、分子改造到抗体表达生产工艺和成药性评估的抗体药物开发技术平台，从而推进基础研究中获得的创新性治疗性抗体向产业转化。

4.以细胞囊泡化为基础通过抗体引导靶向递送抗肿瘤药物研究。

发表论文（*Corresponding author）

1.Wen GP, He L, Tang ZM, Wang SL, Zhang X, Chen YZ, Lin X, Liu C, Chen JX, Ying D, Chen ZH, Wang YB, Luo WX, Huang SJ, Li SW, Zhang J, Zheng ZZ, Zhu J, Xia NS. Quantitative evaluation of protective antibody response induced by hepatitis E vaccine in humans. Nat Commun. 2020 Aug 7;11(1):3971. doi: 10.1038/s41467-020-17737-w.

2.Chen H, Chen Y, Deng M, John S, Gui X, Kansagra A, Chen W, Kim J, Lewis C, Wu G, Xie J, Zhang L, Huang R, Liu X, Arase H, Huang Y, Yu H, Luo W, Xia N, Zhang N, An Z, Zhang CC. Antagonistic anti-LILRB1 monoclonal antibody regulates antitumor functions of natural killer cells. J Immunother Cancer. 2020 Aug;8(2):e000515. doi: 10.1136/jitc-2019-000515.

3.Liu X, Liu C, Liu G, Luo W*, Xia N. COVID-19: Progress in diagnostics, therapy and vaccination. Theranostics, 2020; 10(17): 7821-7835

4.Xia L, Zheng ZZ, Liu JY, Chen YJ, Ding JC, Xia NS, Luo WX*, Liu W*. EGFR-targeted CAR-T cells are potent and specific in suppressing triple-negative breast cancer both in vitro and in vivo. Clin Transl Immunology. 2020 May 3;9(5):e01135. doi: 10.1002/cti2.1135.

5.Zhou B, Xia L, Zhang T, You M, Huang Y, He M, Su R, Tang J, Zhang J, Li S, An Z, Yuan Q*, Luo W*, Xia N. Structure guided maturation of a novel humanized anti-HBV antibody and its preclinical development. Antiviral Res. 2020, 11:104757. doi: 10.1016/j.antiviral.2020.104757.

6.Liu X, Liu C, Zheng Z, Chen S, Pang X, Xiang X, Tang J, Ren E, Chen Y, You M, Wang X, Chen X, Luo W*, Liu G*, Xia N*. Vesicular Antibodies: A Bioactive Multifunctional Combination Platform for Targeted Therapeutic Delivery and Cancer Immunotherapy. Adv Mater. 2019, 31(17):e1808294. doi: 10.1002/adma.201808294 

7.Shen C, Zhang M, Chen Y, Zhang L, Wang G, Chen J, Chen S, Li Z, Wei F, Chen J, Yang K, Guo S, Wang Y, Zheng Q, Yu H, Luo W, Zhang J, Chen H, Chen Y, Xia N.An IgM antibody targeting the receptor binding site of influenza B blocks viral infection with great breadth and potency. Theranostics. 2019 Jan 1;9(1):210-231

8.Deng M, Gui X, Kim J, Xie L, Chen W, Li Z, He L, Chen Y, Chen H, Luo W, Lu Z, Xie J, Churchill H, Xu Y, Zhou Z, Wu G, Yu C, John S, Hirayasu K, Nguyen N, Liu X, Huang F, Li L, Deng H, Tang H, Sadek AH, Zhang L, Huang T, Zou Y, Chen B, Zhu H, Arase H, Xia N, Jiang Y, Collins R, You MJ, Homsi J, Unni N, Lewis C, Chen GQ, Fu YX, Liao XC, An Z, Zheng J, Zhang N, Zhang CC. LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration.Nature. 2018 Oct;562(7728):605-609.

9.Zhou B, Xu L, Zhu R, Tang J, Wu Y, Su R, Yin Z, Liu D, Jiang Y, Wen C, You M, Dai L, Lin Y, Chen Y, Yang H, An Z, Fan C*, Cheng T*, Luo W*, Xia N. A bispecific broadly neutralizing antibody against enterovirus 71 and coxsackievirus A16 with therapeutic potential. Antiviral Res. 2019,161:28-35

10.Zhang C, Huang X, Chen S, Li Y, Li Y, Wang X, Tang J, Xia L, Lin Z, Luo W, Li T, Li S, Zhang J, Xia N, Zhao Q. Epitope clustering analysis for vaccine-induced human antibodies in relationship to a panel of murine monoclonal antibodies against HPV16 viral capsid. Vaccine. 2018 Oct 29;36(45):6761-6771

11.You M, Yang Y, Zhong C, Chen F, Wang X, Jia T, Chen Y, Zhou B, Mi Q, Zhao Q, An Z, Luo W*, Xia N. Efficient mAb production in CHO cells with optimized signal peptide, codon, and UTR.Appl Microbiol Biotechnol. 2018 May 8. doi: 10.1007/s00253-018-8986-5.

12.Yang Y, You M, Chen F, Jia T, Chen Y, Zhou B, Mi Q, An Z, Luo W*, Xia N*. Efficient Development of a Stable Cell Pool for Antibody Production Using a Single Plasmid. J Biochem. 2018,163(5):391-398

13.Kang C, Xia L, Chen Y, Zhang T, Wang Y, Zhou B, You M, Yuan Q, Tzeng CM, An Z, Luo W*, Xia N. A novel therapeutic anti-HBV antibody with increased binding to human FcRn improves in vivo PK in mice and monkeys. Protein Cell. 2018, 9(1):130-134.

14.Wang X, An Z, Luo W, Xia N, Zhao Q*. Molecular and functional analysis of monoclonal antibodies in support of biologics development. Protein Cell. 2018 Jan;9(1):74-85.

15.Xia L, Su R, An Z, Fu TM, Luo W*.  Human Cytomegalovirus vaccine development: Immune responses to look into vaccine strategy. Human Vaccines & Immunotherapeutics..2018,14(2):292-303.

16.Xia L, Tang A, Meng W, Freed DC, He L, Wang D, Li F, Li L, Xiong W, Gui X, Schultz RD, Chen H, He X, Swoyer R, Ha S, Liu Y, Morris CD, Zhou Y, Wang IM, Zhao Q, Luo W, Xia N, Espeseth AS, Hazuda DJ, Rupp RE, Barrett AD, Zhang N, Zhu J, Fu TM, An Z. Active evolution of memory B-cells specific to viral gH/gL/pUL128/130/131 pentameric complex in healthy subjects with silent human cytomegalovirus infection. Oncotarget. 2017, 8(43):73654-73669

17.Shen C, Chen J, Li R, Zhang M, Wang G, Stegalkina S, Zhang L, Chen J, Cao J, Bi X, Anderson SF, Alefantis T, Zhang M, Cai X, Yang K, Zheng Q, Fang M, Yu H, Luo W, Zheng Z, Yuan Q, Zhang J, Wai-Kuo Shih J, Kleanthous H, Chen H, Chen Y, Xia N.A multimechanistic antibody targeting the receptor binding site potently cross-protects against influenza B viruses.Sci Transl Med. 2017 Oct 18;9(412). pii: eaam5752. doi: 10.1126/scitranslmed.aam5752.

18.Xia L, Xian Y, Wang D, Chen Y, Huang X, Bi X, Yu H, Fu Z, Liu X, Li S, An Z, Luo W*, Zhao Q, Xia N*. A human monoclonal antibody against HPV16 recognizes an immunodominant and neutralizing epitope partially overlapping with that of H16.V5. Sci Rep. 2016, 6:19042. doi: 10.1038/srep19042.

19.Zhang TY, Yuan Q, Zhao JH, Zhang YL, Yuan LZ, Lan Y, Lo YC, Sun CP, Wu CR, Zhang JF, Zhang Y, Cao JL, Guo XR, Liu X, Mo XB, Luo WX, Cheng T, Chen YX, Tao MH, Shih JW, Zhao QJ, Zhang J, Chen PJ, Yuan YA, Xia NS. Prolonged suppression of HBV in mice by a novel antibody that targets a unique epitope on hepatitis B surface antigen. Gut, 2015.doi:10.1136/gutjnl-2014-308964.

20.Xia L, Zhang J, Cui C, Bi X, Xiong J, Yu H, An Z, Luo W*, Xia N*. In vitro affinity maturation and characterization of anti-P24 antibody for HIV diagnostic assay. J Biochem. 2015, 158(6):531-538.

21.Tao Jiang, Daiping Guo, Qian Wang, Xin Wu, Zhao Li, Zhenhua Zheng, Boyuan Yin, Lin Xia, Jixian Tang, Wenxin Luo, Ningshao Xia, Yunbao Jiang. Developing a Genetically Encoded Green Fluorescent Protein Mutant for Sensitive Light-up Fluorescent Sensing and Cellular Imaging of Hg(II). Anal Chim Acta. 2015，876: 77-82

22.You M, Xin L, Yang Y, Zhang X, Chen Y, Yu H, Li S, Zhang J, An Z, Luo W*, Xia N*. Investigation of a special neutralizing epitope of HEV E2s. Protein Cell. 2014 Dec;5(12):950-3.

23.You M, Liu Y, Chen Y, Guo JY, Wu J, Fu Y, Shen R, Qi R, Luo W*, Xia N*. Maximizing antibody production in suspension-cultured mammalian  cells by customized transient gene expression method. Biosci. Biotech. Bioch, 77(6):1207-1213,2013.

24.Chen Y, Luo W*, Song H, Yin B, Tang J, Chen Y, Ng MH, Yeo A, Zhang J, Xia N*. Mimotope ELISA for Detection of Broad Spectrum Antibody against Avian H5N1 Influenza Virus. Plos One. 6(9): e24144, 2011  

25.Zheng Q, Xia L, Wu WL, Zheng Z, Huo Y, Wu J, Liu Y, Yu H, Chen Y, Lau SY, Chen H*, Luo W*, Xia N. Properties and therapeutic efficacy of broadly reactive chimeric and humanized H5-specific monoclonal antibodies against H5N1 influenza viruses. Antimicrob Agents Chemother. 55(4):1349-1357, 2011

26.Zheng Z, Luo W*, Song H, Chen Y, Xiong J, Chen Y, Gu Y, Chen H, Shih JW, Zhang J, Xia N*. Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins. Arch Virol. 155(1):19-26, 2010 Song H, Luo W*, Chen Y, Du H, Tang J, Yin B, ChenY, Shi JW, Zhang J. Induction of cross-reactive antibodies against mimotopes of H5N1 hemagglutinin. Vet. Microbiol, 145(1-2):17-22, 2010

27.Chen Y, Luo W*, Wu WL, Fang Z, Xia L, Gui X, Chen Y, Chen H, Shih JW, Xia N*. Humanized antibodies with broad-spectrum neutralization to avian influenza virus H5N1.Antiviral Res. 87(1):81-88, 2010

28.Luo W*, Chen Y, Wang M, Chen Y, Zheng Z, Song H, Chen H, Guan Y, Ng MH, Zhang J, Xia N. Peptide mimics of a conserved H5N1 avian influenza virus neutralization site. Biochem J. 419:133-139, 2009

29.Luo W, Chen Y, Li L, Xu C, Miao J, Shih JW, Zhang J, Xia N. Construction and characterization of the chimeric antibody8C11 to the hepatitis E virus. FEMS Immunol Med icrobiol. 51(1):18-25, 2007

30.Chen Y, Luo W, Wang M, Wang J, Li L, Yuan Q, Zhang J, Xia N. Isolation of human antibodies against hepatitis E virus from phage display library by immobilized metal affinity chromatography. Biomedical and Environmental Sciences 2007. 20:488-494.

31.Luo W, Cheng T, Guan B, Li S, Zhang J, Xia N. Variants of Green Fluorescent Protein GFPxm. Marine Biotech. 8:560-566, 2006

奖励

1.张军，王颖彬，尹红章，彭耿，夏宁邵，张国忠，罗文新，程通，吴婷，李秀华. 人类T淋巴细胞白血病病毒重组抗原及抗体诊断试剂盒的研制。厦门市科技进步，二等奖，2004年。教育部科技进步，二等奖，2006年。

2.2015年作为科研团队成员之一荣获求是科技基金会“杰出科技成就集体奖”。

3.作为核心成员之一所在的“疫苗与体外诊断创新团队”入选为2013年科技部创新人才推进计划重点领域创新团队。

承担的科研课题

1.功能化双特异性抗体囊泡用于肝癌免疫治疗（32070940），国家自然科学基金，2021-2024，课题负责人  

2.新冠中和抗体  厦门市科技项目（2020.1-12），课题负责人  

3.重大传染病防治药物 ，地市级科技项目，2019-2021，课题负责人  

4.高效持久清除乙肝病毒的新型抗体研究(31870925),国家自然科学基金，2019-2022，课题负责人    

5.基于Anti-HBV抗体的乙型肝炎治愈新方法（2017ZX10202203）,传染病重大专项，2017-2020,子课题负责人   

6.人巨细胞病毒自然感染过程中体液免疫应答机制研究（31670927），国家自然科学基金，2017-2020，课题负责人   

7.心血管疾病相关管式化学发光定量测定试剂研制（3502Z20140050），厦门市科技计划项目，2015-2017，课题负责人  

8.乙肝表面抗原清除的新策略新机制研究（2013ZX10002002），传染病重大专项，2013-2015,子课题负责人  

9.H5禽流感病毒抗原快速检测诊断试剂盒的研制（2008ZX10004-006）,传染病重大专项，2008-2011，子课题负责人  

10.H5N1禽流感病毒血凝素蛋白重要中和表位的初步研究（108157），教育部科学技术研究重点项目，2007－2009，课题负责人  

11.高致病性H5N1禽流感病毒血凝素抗原位点图研究（30640017），国家自然科学基金，2007.1-12，课题负责人