顾颖，博士 GU Ying, Ph.D.
副教授，硕士生导师 Associate Professor
电话：+86-592-2187880
传真：+86-592-2181258
E-mail：guying@xmu.edu.cn
简历 (Education and Work experience)
1996年，厦门大学生物学专业，学士学位；
2006年，厦门大学生化与分子生物学专业/细胞生物学专业，博士学位；
2006年，厦门大学生命科学学院助理教授、硕士生导师；
2013年，厦门大学生命科学学院副教授；
B.S. Biochemistry, Xiamen University, 2000
Ph.D. Biochemistry & Molecular Biology/Cell Biology, Xiamen University, 2006
Assistant Professor, School of Life Sciences, Xiamen University, 2006
Associate Professor, School of Life Sciences, Xiamen University, 2013
研究领域 (Research Area)
1、抗病毒的单抗筛选：利用细胞模型和重组抗原等进行抗病毒的单抗筛选，如HIV中和单抗、识别免疫优势表位单抗等的筛选。
2、基于表位的抗原研究：利用基因工程重组技术表达获得含有功能表位的抗原或颗粒化抗原。
3、功能性表位研究：应用生物传感器、分析超离、微量热等方法研究抗原-抗体的相互作用，尝试解析免疫复合物的空间结构，鉴定功能性表位的特征。
4、Generation of monoclonal antibodies against different viral targets : based on functional screening by virus-infection cell model, recombinant antigen reactivity and et. al, focusing on HIV neutralizing monoclonal antibodes, esp. those with broad neutralizing activity
5、Identification and characterization of key epitopes on viral antigens: Design and preparation of recombinant antigens harbouring functional epitopes
6、Functional characterization of epitopes: epitope mapping, structural analysis and function interpretation by various methods including SPR-based assay, analytical ultracentrifugation, microcalorimetry, and structural determination of antibody-antigen complexes
代表性论文(Selected Publications)(* Corresponding author)
1、Gu Y, Tang X, Zhang X, Song C, Zheng M, Wang K, Zhang J, Ng MH, Hew CL, Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody. Cell Res. 2015 May;25(5):604-20.
2、Wang D, Li Z, Xiao J, Wang J, Zhang L, Liu Y, Fan F, Xin L, Wei M, Kong Z, Yu H, Gu Y, Zhang J, Li S, Xia N. Identification of Broad-Genotype HPV L2 Neutralization Site for Pan-HPV Vaccine Development by a Cross-Neutralizing Antibody. PLoS One. 2015 Apr 23;10(4):e0123944.
3、Gu Y, Cao F, Wang L, Hou W, Zhang J, Hew CL, Li S*, Yuan YA*, Xia N*. Structure of a Novel Shoulder-to-Shoulder p24 Dimer in Complex with the Broad-Spectrum Antibody A10F9 and Its Implication in Capsid Assembly. PLoS One. 2013 Apr 19;8(4):e61314.
4、Yang C, Pan H, Wei M, Zhang X, Wang N, Gu Y, Du H, Zhang J,Li S*, Xia N*.Hepatitis Evirus capsid protein assembles in 4M urea in the presence of salts.Protein Sci. 2013 Mar;22(3):314-26.
5、Gu Y, Hou W, Xu C, Li S, Shih JW, Xia N*. The enhancement of RNAi against HIV in vitro and in vivo using H-2K(k) protein as a sorting method. J Virol Methods. 2012 Jun;182(1-2):9-17.
6、Shen L1, Gu Y 1, Sun L, Yang Y, Wang F, Li Y, Bi S*. Development of a hepatitis delta virus antibody assay for study of the prevalence of HDV among individuals infected with hepatitis B virus in China. J Med Virol. 2012 Mar;84(3):445-9. (co-1st)
7、Tang X, Yang C, Gu Y, Song C, Zhang X, Wang Y, Zhang J, Hew CL,Li S*, Xia N*, Sivaraman J*. Structural basis for the neutralization and genotype specificity of hepatitis E virus. ProcNatl Acad Sci U S A. 2011 Jun 21;108(25):10266-71.
8、Yu H,Li S*, Yang C, Wei M, Song C, Zheng Z, Gu Y, Du H, Zhang J, Xia N*. Homology model and potential virus-capsid binding site of a putative HEV receptor Grp78. J Mol Model. 2011 May;17(5):987-95.
9、Zheng Z, Luo W*, Song H, Chen Y, Xiong J, Chen Y, Gu Y, Chen H, Shih JW, Zhang J, Xia N*. Antibody reactivity of conformational peptide mimics of a conserved H5N1 neutralization site in different fusion proteins. Arch Virol. 2010;155(1):19-26.
10、Li S, Tang X, Seetharaman J, Yang C, Gu Y, Zhang J, Du H, Shih JW, Hew CL, Sivaraman J*, Xia N*. Dimerization of Hepatitis E Virus Capsid Protein E2s Domain is Essential for Virus-Host Interaction.PLoS Pathog. 2009 Aug;5(8):e1000537.
11、Xu CY, Gu Y*, Hou WH, Que YQ, Gao SG, Cheng T, Xia NS*. Tetracysteine as a Reporter for Gene Therapy. Biomed Environ Sci. 2009 Dec;22(6):496-501.
12、Li S, Zhang J, He Z, Gu Y, Liu R, Lin J, Chen Y, Ng M, Xia N*. Mutational analysis of essential interactions involved in the assembly of hepatitis E virus capsid.J Biol Chem. 2005 Feb 4;280(5):3400-6.
13、Yang HJ, Chen M, Cheng T, He SZ, Li SW, Guan BQ, Zhu ZH, Gu Y, Zhang J, Xia NS.Expression and immunoactivity of chimeric particulate antigens of receptor binding site-core antigen of hepatitis B virus. World J Gastroenterol. 2005 Jan 28;11(4):492-7.
14、Zhang J, Gu Y, Ge SX, Li SW, He ZQ, Huang GY, Zhuang H, Ng MH, Xia NS. Analysis of hepatitis E virus neutralization sites using monoclonal antibodies directed against a virus capsid protein. Vaccine. 2005 Apr 22;23(22):2881-92.
15、Gu Y, Zhang J, Wang YB, Li SW, Yang HJ, Luo WX, Xia NS*. Selection of a peptide mimicking neutralization epitope of hepatitis E virus with phage peptide display technology. World J Gastroenterol. 2004 Jun 1;10 (11):1583-8.
16、Zhang J, Ge SX, Huang GY, Li SW, He ZQ, Wang YB, Zheng YJ, Gu Y, Ng MH, Xia NS. Evaluation of antibody-based and nucleic acid-based assays for diagnosis of hepatitis E virus infection in a rhesus monkey model. J Med Virol. 2003 Dec;71(4):518-26.
荣誉、奖励及参加学术团体的情况(Honors and award)
主持3项国家自然科学基金项目、1项国家科技重大专项子课题，1项福建省科技重大项目，并作为主要参与人参加多项国家级、省部级重大重点科研项目；获得中国高校科技进步一等奖（2001），国家技术发明二等奖（2010），中国专利金奖（2012），厦门市科学技术进步一等奖（2010），厦门大学第四届青年教师教学技能比赛一等奖（2008），厦门大学第三届青年教师教学技能比赛二等奖（2007）。申请发明专利11项，国际发明专利8项，获得授权4项；为主参与研制戊肝疫苗、宫颈癌疫苗、艾滋诊断试剂等新药。
WIPO-SIPO Award for Chinese Outstanding Patented Invention (2012)
State Technological Invention Award (2010)
